DIYbio:Notebook/Open Thermal Cycler/Requirements: Difference between revisions
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In progress... | |||
Use cases of thermal cyclers: | |||
# | |||
# | |||
# A user | |||
The Pain of Current Thermal Cyclers: | |||
Price - thermal cyclers | |||
Interface - current models are | |||
Application - | |||
The ideal solution: | |||
A user places their sample into Device X. The user picks Device X replicates a desired sequence of DNA. | |||
Problems the Open Thermal Cycler solves: | |||
Price - | |||
High Level Requirements | |||
#The Open Thermal Cycler (OTC) shall allow users to replicate DNA | |||
#"" software must allow users to load settings manually | |||
#"" hardware must support a range of temperatures from X to Y | |||
#"" may be accessible through a web interface | |||
#"" may allow users to load pre-configured settings | |||
#"" may allow users to know if the machin | |||
#Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline | #Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline | ||
Response: Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used. Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers | Response: Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used. Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers | ||
Revision as of 23:08, 23 April 2009
In progress...
Use cases of thermal cyclers:
- A user
The Pain of Current Thermal Cyclers: Price - thermal cyclers Interface - current models are Application -
The ideal solution:
A user places their sample into Device X. The user picks Device X replicates a desired sequence of DNA.
Problems the Open Thermal Cycler solves: Price -
High Level Requirements
- The Open Thermal Cycler (OTC) shall allow users to replicate DNA
- "" software must allow users to load settings manually
- "" hardware must support a range of temperatures from X to Y
- "" may be accessible through a web interface
- "" may allow users to load pre-configured settings
- "" may allow users to know if the machin
- Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline
Response: Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used. Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers
