DIYbio:Notebook/Open Thermal Cycler/Requirements

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Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline
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#Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline
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Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used.  Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers
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Response: Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used.  Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers
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When you feel like just clicking, use your "Biobricks" profile that you download from igem.org. It's preconfigured to the Taq you're using. Click the profile and go! The same with the "Bioweather Maps" profile that you get from Jason Bobe -- amplify ribosomal RNA. Or, if you're amplifying another sequence, you copy/paste in your primer sequences and the PCR machine does the rest of the calculations. -- Tito Jankowski
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Revision as of 01:26, 15 April 2009

  1. Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline

Response: Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used. Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers

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