DIYbio:Notebook/Open Thermal Cycler/Requirements

1. Perhaps the operator doesn't know (or care) how the machine is actually cycling - if the protocol has been verified, the task is to run it, get the result & move on. -- Jonathan Cline

Response: Many PCRs are more complicated than that unfortunately, the melting temperature (Tm) of the primer-DNA is just an estimate and often the annealing temp. needs adjusting with each template and primer used. Likewise the extension time is also often adjusted depending on the product length in order to speed up the total time it takes to run, -- John Cumbers