DMPC
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| - | + | [[Image:MolecularFormulaDMPC.gif|thumb|dimethyl pyrocarbonate]] | |
| - | DMPC | + | DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester). |
| - | + | ==Purpose== | |
| + | *DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses. | ||
| - | DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way. | + | ==Procurement== |
| + | *You can buy DMPC for example at [http://www.sigmaaldrich.com Sigma]. | ||
| + | |||
| + | ==Use== | ||
| + | *DMPC is a safer alternative to [[DEPC]] (known carcinogen). It is used in exactly the same way. | ||
| + | |||
| + | *Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC). | ||
| + | |||
| + | *Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution. | ||
| + | |||
| + | ==Safety== | ||
| + | |||
| + | *DMPC is a safer alternative to [[DEPC]] (known carcinogen). | ||
==References== | ==References== | ||
#[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA] | #[http://stanxterm.aecom.yu.edu/wiki/index.php?page=RNA_handling Handling RNA] | ||
#[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth] | #[http://www.ambion.com/techlib/tb/tb_178.html RNase and DEPC Treatment: Fact or Laboratory Myth] | ||
| + | |||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:RNA]] | [[Category:RNA]] | ||
| + | [[Category:Chemical]] | ||
Current revision
DMPC is an acronym for di-methyl pyrocarbonate (AKA: dimethyl dicarbonate, pyrocarbonic acid dimethyl ester).
Contents |
Purpose
- DMPC is typically used to treat water to remove RNases. It reacts with amine, hydroxy and thiol groups of proteins (such as RNAses) and inactivates RNAses.
Procurement
- You can buy DMPC for example at Sigma.
Use
- DMPC is a safer alternative to DEPC (known carcinogen). It is used in exactly the same way.
- Treatment involves adding DMPC to 0.1% v/v and incubating at 37°C for 1 hour to overnight followed by autoclaving. Autoclaving destroys DMPC and is an essential step. Esters may be generated during autoclaving giving rise to a 'fruity' smell (that is not coming directly from DMPC).
- Note that DMPC cannot be used with chemical solutions that have amine groups, such as Tris and HEPES buffers, or mercaptans. In such cases, use DMPC-treated water to generate the solution.
Safety
- DMPC is a safer alternative to DEPC (known carcinogen).
