DNA Precipitation

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(New page: ==Overview== Replace this sentence with a brief description of the protocol and its goal. ==Materials== List reagents, supplies and equipment necessary to perform the protocol here. For...)
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List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
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*supply 1 (i.e. tubes of a certain size? spreaders?)
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*3M NaOAc pH 5.2
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*reagent 1
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*EtOH 95%
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*X μL reagent 2
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*Glycogen (optional)
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**component A (reagent 2 is made up of multiple components)
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**component B
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*equipment 1
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*equipment 2
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==Procedure==
==Procedure==
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#Step 1
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#1/10 volume NaOAc
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#Step 2
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#2 volumes EtOH
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#*Step 2 has some additional information that goes with it.  i.e. Keep at 4°C.
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#-20°C overnight or 30min -70°C.
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#Step 3
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#Centrifuge >12k G for at least 15 mins.
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##Step 3 has multiple sub-steps within it.
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##Enumerate each of those.
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==Notes==
==Notes==

Revision as of 17:10, 26 July 2007

Contents

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • 3M NaOAc pH 5.2
  • EtOH 95%
  • Glycogen (optional)

Procedure

  1. 1/10 volume NaOAc
  2. 2 volumes EtOH
  3. -20°C overnight or 30min -70°C.
  4. Centrifuge >12k G for at least 15 mins.


Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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