DNA Precipitation

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(Procedure)
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==Procedure==
==Procedure==
-
#1/10 volume NaOAc
+
#1/10 volume NaOAc.
-
#2 volumes EtOH
+
#1ul Glycogen.
 +
#2 volumes EtOH.
#-20°C overnight or 30min -70°C.
#-20°C overnight or 30min -70°C.
#Centrifuge >12k G for at least 15 mins.
#Centrifuge >12k G for at least 15 mins.
-
 
==Notes==
==Notes==

Revision as of 16:11, 26 July 2007

Contents

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • 3M NaOAc pH 5.2
  • EtOH 95%
  • Glycogen (optional)

Procedure

  1. 1/10 volume NaOAc.
  2. 1ul Glycogen.
  3. 2 volumes EtOH.
  4. -20°C overnight or 30min -70°C.
  5. Centrifuge >12k G for at least 15 mins.

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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