DNA Precipitation

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(Procedure)
(Procedure)
Line 14: Line 14:
#1ul Glycogen.
#1ul Glycogen.
#2 volumes EtOH.
#2 volumes EtOH.
-
#-20°C overnight or 30min -70°C.
+
#-20°C overnight or 30min -80°C.
 +
-20°C for one hour is plenty for large quantities (1mL bacterial culture, plasmid) of DNA.
#Centrifuge >12k G for at least 15 mins.
#Centrifuge >12k G for at least 15 mins.
 +
#Remove supernatant
 +
#Resuspend in desired volume of water/buffer
==Notes==
==Notes==

Revision as of 16:11, 22 April 2008

Contents

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that page. Alternatively, links to the suppliers' page on that material are also appropriate.

  • 3M NaOAc pH 5.2
  • EtOH 95%
  • Glycogen (optional)

Procedure

  1. 1/10 volume NaOAc.
  2. 1ul Glycogen.
  3. 2 volumes EtOH.
  4. -20°C overnight or 30min -80°C.

-20°C for one hour is plenty for large quantities (1mL bacterial culture, plasmid) of DNA.

  1. Centrifuge >12k G for at least 15 mins.
  2. Remove supernatant
  3. Resuspend in desired volume of water/buffer

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.


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