DNA Precipitation

Overview

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Materials

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• 3M NaOAc pH 5.2
• EtOH 95%
• Glycogen (optional)

Procedure

1. 1/10 volume NaOAc.
2. 1ul Glycogen.
3. 2 volumes EtOH.
4. -20°C overnight or 30min -80°C.
5. Centrifuge >12k G for at least 15 mins.
6. Remove supernatant
7. Resuspend in desired volume of water/buffer

Notes

'''*~~~~''': -20°C for an hour is fine for using larger (1mL of bacterial culture, plasmid) amounts of DNA

The DNA pellet will not always be visible depending on how much DNA you are precipitating. So always take care in loading your samples in the centrifuge to remember the direction they are facing. The DNA pellet will be on the part of the tube facing the outside of the centrifuge.

References

Relevant papers and books

1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
3. ISBN:0879697164

Contact

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