DNA Precipitation protocol
- 3M Sodium Acetate solution
- 95% EtOH
- 70% EtOH
- DNA sample
- Eppendorf tubes
- Measure out DNA sample into an Eppendorf tube.
Add 0.1 volume 3M Sodium Acetate solution.
- Add 1 µl of Glycogen.
- Add 2 volumes 95% EtOH.
Option 1: Store at -20°C for 12 hrs(overnight).
Option 2: Store at -80°C for 30 mins.
- Centrifuge at maximum speed for at least 15 mins at room temperature, gently aspirate out the supernatant and discard it.
- Dry the pellet in air for 10 - 15 mins.
Dry until all the liquid is gone.
Option 1: Add water to pellet.
Option 2: Add buffer to pellet.
Resuspend the pellet by vortexing/by shaking vigorously.
Add 1 ml of 70% EtOH.
Store at room temperature for 5 mins.
Centrifuge at maximum speed for 5 mins at room temperature, gently aspirate out the supernatant and discard it.