DNA blot (Southern)
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The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling. | The '''DNA blot''' or '''Southern blot''' is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a [[restriction endonuclease]]. Fragments are then separated by size using [[agarose gel electrophoresis]]. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling. | ||
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| + | == Lab-specific protocols == | ||
| + | * [[Oneill_Lab:Southern_Blot]] | ||
== See also == | == See also == | ||
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* [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video] | * [http://www.youtube.com/watch?v=zYSPnSwT6LQ Principle of DNA blot explained in a video] | ||
* [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson] | * [http://www.bio.davidson.edu/COURSES/GENOMICS/method/Southernblot.html DNA blot overview at Davidson] | ||
| + | * [http://www.dmd.nl/protocol/DNAhybridization_ALG005.html detailed DNA blot protocol from Leiden University] | ||
* [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia] | * [http://en.wikipedia.org/wiki/Southern_blot DNA blot at Wikipedia] | ||
* [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia] | * [http://en.wikipedia.org/wiki/Electroblotting Electroblotting at Wikipedia] | ||
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| + | [[Category:Protocol]] | ||
| + | [[Category:DNA]] | ||
| + | [[Category:in vitro]] | ||
Current revision
The DNA blot or Southern blot is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA is first digested using a restriction endonuclease. Fragments are then separated by size using agarose gel electrophoresis. After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The location of the probe on the membrane is visualised either by radioactivity or antibody labelling.
