DNA ladder
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*[http://www.neb.com/ NEB]'s [http://www.neb.com/nebecomm/products/productN3200.asp 2-log ladder] is a good choice for most samples. | *[http://www.neb.com/ NEB]'s [http://www.neb.com/nebecomm/products/productN3200.asp 2-log ladder] is a good choice for most samples. | ||
*Deionized Water | *Deionized Water | ||
| - | *Loading dye | + | *[[Loading dye]] |
| - | Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add | + | Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA. |
| + | |||
| + | To make 1.2 mL prepared ladder, combine: | ||
| + | #900μL deionized water | ||
| + | #100μL 2-log ladder (at 1μg/μL) | ||
| + | #200μL loading dye | ||
| + | |||
| + | |||
| + | [[Category:Material]] [[Category:Agarose gel electrophoresis]] | ||
Current revision
Prepared DNA ladder
- NEB's 2-log ladder is a good choice for most samples.
- Deionized Water
- Loading dye
Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.
To make 1.2 mL prepared ladder, combine:
- 900μL deionized water
- 100μL 2-log ladder (at 1μg/μL)
- 200μL loading dye
