DNA ladder: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(add tags) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 3: | Line 3: | ||
*[http://www.neb.com/ NEB]'s [http://www.neb.com/nebecomm/products/productN3200.asp 2-log ladder] is a good choice for most samples. | *[http://www.neb.com/ NEB]'s [http://www.neb.com/nebecomm/products/productN3200.asp 2-log ladder] is a good choice for most samples. | ||
*Deionized Water | *Deionized Water | ||
*Loading dye | *[[Loading dye]] | ||
Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add | Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA. | ||
To make 1.2 mL prepared ladder, combine: | |||
#900μL deionized water | |||
#100μL 2-log ladder (at 1μg/μL) | |||
#200μL loading dye | |||
[[Category:Material]] [[Category:Agarose gel electrophoresis]] | |||
Latest revision as of 08:34, 11 March 2008
Prepared DNA ladder
- NEB's 2-log ladder is a good choice for most samples.
- Deionized Water
- Loading dye
Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.
To make 1.2 mL prepared ladder, combine:
- 900μL deionized water
- 100μL 2-log ladder (at 1μg/μL)
- 200μL loading dye
