DNA polymerase
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(New page: ==Characteristics== ===Extension rate=== *How rapidly a particular DNA polymerase enzyme synthesizes a complementary strand. ===Processivity=== *A measure of how strongly the polymerase bi...) |
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| + | ==Specific polymerases== | ||
| + | <!--To list a polymerase here, "tag" the protocol page with the [[Category:Material]] and [[Category:Enzyme]] and [[Category:Polymerase]] and [[Category:DNA]]--> | ||
| + | {{#dpl:category=Material|category=Enzyme|category=Polymerase|category=DNA|nottitlematch=DNA polymerase}} | ||
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==Characteristics== | ==Characteristics== | ||
===Extension rate=== | ===Extension rate=== | ||
Current revision
Contents |
Specific polymerases
Characteristics
Extension rate
- How rapidly a particular DNA polymerase enzyme synthesizes a complementary strand.
Processivity
- A measure of how strongly the polymerase binds to template DNA. Processivity doesn't affect extension rate. However, it has a strong influence on the absolute length of complementary DNA strand that can be made.
Proofreading
- Proofreading is when the multi-functional enzyme adds a deoxynucleotide and then goes back and rechecks the template to complement interaction to make sure the correct nucleotide was added.
- Proofreading polymerases are usually multimeric and thus more expense to make.
- Like all DNA polymerases, proofreading polymerases add an adenine deoxynucleotide to the 3' end of the complementary strand. Proofreading ensures that this nucleotide is removed again leaving blunt-ended DNA.
