DNA separation in different agarose gels: Difference between revisions
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[[Image:DNA gel agarose EtBr by UV.jpg|thumb|'''Figure 1.''' Bands separation in 1 kbp and 100 bps DNA ladder are shown in different agarose gel concentration. Gels are illuminated under UV light condition shortly after 20 minutes running time.|300px]] | |||
This experiment was aimed to show the different band separation in 1%, 2% and 3% DNA agarose gels. 1 kbp and 100 bp DNA ladders were used and run at the same time for 20 minutes at 100 Volts (~400 mA). The distance of bands traveling in the gels were compared. Ladder bands, especially the larger DNA molecules, were shifting towards the top of the gel as agarose concentration was increased. | |||
== 1 kbp and 100 bp in different agarose gels == | |||
[ | [[Image:1_kb_DNA_ladder.jpg|300px]] [[Image:100 bp DNA ladder.jpg|300px]] | ||
'''Figure 2'''. 1 kbp DNA ladder (NEB, 250 ng, left) and 100 bp DNA ladder (NEB, 500 ng, right) band separation in different agarose gels (concentration 1, 2, 3%) run for the same time at 100V in TAE buffer. | |||
* [http://www.neb.com/nebecomm/products/productn3232.asp 1kbp DNA ladder by NEB] | |||
* [http://www.neb.com/nebecomm/products/productN3231.asp 100 bp DNA ladder by NEB] | |||
== See also == | == See also == | ||
* [[Agarose gel electrophoresis]] | * [[Agarose gel electrophoresis]] | ||
[[Category:DNA]] | |||
[[Category:Agarose gel electrophoresis]] | |||
Latest revision as of 03:00, 25 September 2009
This experiment was aimed to show the different band separation in 1%, 2% and 3% DNA agarose gels. 1 kbp and 100 bp DNA ladders were used and run at the same time for 20 minutes at 100 Volts (~400 mA). The distance of bands traveling in the gels were compared. Ladder bands, especially the larger DNA molecules, were shifting towards the top of the gel as agarose concentration was increased.
1 kbp and 100 bp in different agarose gels
Figure 2. 1 kbp DNA ladder (NEB, 250 ng, left) and 100 bp DNA ladder (NEB, 500 ng, right) band separation in different agarose gels (concentration 1, 2, 3%) run for the same time at 100V in TAE buffer.
