DNA separation in different agarose gels: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(leader) |
mNo edit summary |
||
Line 1: | Line 1: | ||
[[Image:DNA_Gel_agarose_cross_sections.jpg|thumb|'''Figure 1.''' Bands separation in 1 kbp and 100 bps DNA ladder are shown in different agarose gel concentration. Gels are illuminated under UV light condition shortly after 20 minutes running time.|300px]] | [[Image:DNA_Gel_agarose_cross_sections.jpg|thumb|'''Figure 1.''' Bands separation in 1 kbp and 100 bps DNA ladder are shown in different agarose gel concentration. Gels are illuminated under UV light condition shortly after 20 minutes running time.|300px]] | ||
This experiment was aimed to show the different band separation in 1%, 2% and 3% DNA agarose gels. 1 kbp and 100 bp DNA ladders were used and run at the same time for 20 minutes at 100 Volts (~400 mA). The distance of bands traveling in the gels were compared. Ladder bands, especially the larger DNA molecules, were shifting towards the top of the gel as agarose concentration was increased. | |||
== 1 kbp in different agarose gels == | == 1 kbp in different agarose gels == |
Revision as of 02:51, 25 September 2009
This experiment was aimed to show the different band separation in 1%, 2% and 3% DNA agarose gels. 1 kbp and 100 bp DNA ladders were used and run at the same time for 20 minutes at 100 Volts (~400 mA). The distance of bands traveling in the gels were compared. Ladder bands, especially the larger DNA molecules, were shifting towards the top of the gel as agarose concentration was increased.
1 kbp in different agarose gels
100 bp in different agarose gels