DNA separation in different agarose gels

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(categories)
Current revision (05:00, 25 September 2009) (view source)
(See also)
 
Line 16: Line 16:
[[Category:DNA]]
[[Category:DNA]]
-
[[Category:agarose]]
+
[[Category:Agarose gel electrophoresis]]

Current revision

Figure 1. Bands separation in 1 kbp and 100 bps DNA ladder are shown in different agarose gel concentration. Gels are illuminated under UV light condition shortly after 20 minutes running time.
Figure 1. Bands separation in 1 kbp and 100 bps DNA ladder are shown in different agarose gel concentration. Gels are illuminated under UV light condition shortly after 20 minutes running time.

This experiment was aimed to show the different band separation in 1%, 2% and 3% DNA agarose gels. 1 kbp and 100 bp DNA ladders were used and run at the same time for 20 minutes at 100 Volts (~400 mA). The distance of bands traveling in the gels were compared. Ladder bands, especially the larger DNA molecules, were shifting towards the top of the gel as agarose concentration was increased.

1 kbp and 100 bp in different agarose gels

Figure 2. 1 kbp DNA ladder (NEB, 250 ng, left) and 100 bp DNA ladder (NEB, 500 ng, right) band separation in different agarose gels (concentration 1, 2, 3%) run for the same time at 100V in TAE buffer.

See also

Personal tools