DOs and DONTs of Good Lab Citizenship

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* Stay SAFE!
* Stay SAFE!
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* Be CONSIDERATE of others (their stuff, needs, space, etc)
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Be CONSIDERATE of others (their stuff, needs, space, etc)  
-
* Always ASK SOMEONE if you’re not sure about something.
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Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.
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* THINK before you do.
+
THINK before you do.
-
* CLEAN UP after yourself.
+
CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.
-
* Maintain a CLEAN workspace.
+
Maintain a CLEAN workspace.
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* Maintain STERILE technique.
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∑ REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)
-
* ASK someone before you move their stuff.
+
∑ If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.
-
* BALANCE the [[Knight:5810R_centrifuge|centrifuges]].
+
Maintain STERILE technique.
-
* ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
+
ASK someone before you move their stuff.
-
* If you use the rotator in the 37C room, remember to TURN IT BACK ON
+
∑ Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.
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* CLOSE refrigerator doors!!
+
BALANCE the centrifuges.
-
* Use YOUR OWN [[Endy:Making_1%25_Agarose_stock|master stocks]] of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
+
ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
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* READ [[Protocols|PROTOCOLS]] before you attempt them.
+
If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.
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* Keep any proteins ( [[Materials|endonucleases]], [[DNA_ligation|ligase]], [[Smolke:Taq_Buffer|Taq supermix]]) always ON ICE
+
CLOSE refrigerator doors!!
-
* CHANGE TIPS between aliquots of important stocks.
+
Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
-
* WEAR GLOVES when working w/ dangerous/sensitive chemicals ([[Phenol|phenol]], RNA, etc).
+
READ PROTOCOLS before you attempt them.  
-
* Keep a good [[Lab_Notebook|LAB NOTEBOOK]] so you know what you did.
+
Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE
-
* LABEL TUBES
+
CHANGE TIPS between aliquots of important stocks.
-
* Store [[Endy:Screening_plasmid/v2.0/Glycerol_box|GLYCEROLS]] appropriately
+
WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).
-
* If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents.
+
Keep a good LAB NOTEBOOK so you know what you did.
 +
LABEL TUBES
 +
Store GLYCEROLS appropriately
 +
If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.
 +
 
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[[image:caution-32x32.png|left]]
[[image:caution-32x32.png|left]]

Revision as of 14:41, 9 June 2008

DO's


  • Stay SAFE!

∑ Be CONSIDERATE of others (their stuff, needs, space, etc) ∑ Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab. ∑ THINK before you do. ∑ CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up. ∑ Maintain a CLEAN workspace. ∑ REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips) ∑ If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder. ∑ Maintain STERILE technique. ∑ ASK someone before you move their stuff. ∑ Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container. ∑ BALANCE the centrifuges. ∑ ASK SOMEONE how to use a piece of equipment if you haven’t used it before. ∑ If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON. ∑ CLOSE refrigerator doors!! ∑ Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use. ∑ READ PROTOCOLS before you attempt them. ∑ Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE ∑ CHANGE TIPS between aliquots of important stocks. ∑ WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc). ∑ Keep a good LAB NOTEBOOK so you know what you did. ∑ LABEL TUBES ∑ Store GLYCEROLS appropriately ∑ If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.

DON'Ts


  • Don’t EVER COMPROMISE SAFETY!
  • Don’t Be too shy to ASK QUESTIONS
  • Don’t Start something without THINKING about what you’re doing
  • Don’t Use a MACHINE without having received training on it.
  • Don’t POUR hazardous or unknown chemicals down the sink.
  • Don’t Leave a door to a cold or warm room OPEN.
  • Don’t IGNORE equipment alarms
  • Don’t USE master stocks other than your own without permission.
  • Don’t Leave your stuff lying around in other people’s areas.
  • Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out).
  • Don’t PLAY MUSIC out loud (not everyone likes your taste).
  • Don’t DATE people you work with.*

* May occasionally result in marriage.


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