DOs and DONTs of Good Lab Citizenship

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* Stay SAFE!
* Stay SAFE!
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Be CONSIDERATE of others (their stuff, needs, space, etc)  
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* Be CONSIDERATE of others (their stuff, needs, space, etc)  
-
Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.
+
* Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.
-
THINK before you do.
+
* THINK before you do.
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CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.
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* CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.
-
Maintain a CLEAN workspace.
+
* Maintain a CLEAN workspace.
-
REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)
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* REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)
-
If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.
+
* If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.
-
Maintain STERILE technique.
+
* Maintain STERILE technique.
-
ASK someone before you move their stuff.
+
* ASK someone before you move their stuff.
-
Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.
+
* Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.
-
BALANCE the centrifuges.
+
* BALANCE the centrifuges.
-
ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
+
* ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
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If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.
+
* If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.
-
CLOSE refrigerator doors!!
+
* CLOSE refrigerator doors!!
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Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
+
* Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
-
READ PROTOCOLS before you attempt them.  
+
* READ PROTOCOLS before you attempt them.  
-
Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE
+
* Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE
-
CHANGE TIPS between aliquots of important stocks.
+
* CHANGE TIPS between aliquots of important stocks.
-
WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).
+
* WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).
-
Keep a good LAB NOTEBOOK so you know what you did.
+
* Keep a good LAB NOTEBOOK so you know what you did.
-
LABEL TUBES
+
* LABEL TUBES
-
Store GLYCEROLS appropriately
+
* Store GLYCEROLS appropriately
-
If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.
+
* If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.
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Revision as of 13:42, 9 June 2008

DO's


  • Stay SAFE!
  • Be CONSIDERATE of others (their stuff, needs, space, etc)
  • Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.
  • THINK before you do.
  • CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.
  • Maintain a CLEAN workspace.
  • REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)
  • If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.
  • Maintain STERILE technique.
  • ASK someone before you move their stuff.
  • Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.
  • BALANCE the centrifuges.
  • ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
  • If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.
  • CLOSE refrigerator doors!!
  • Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
  • READ PROTOCOLS before you attempt them.
  • Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE
  • CHANGE TIPS between aliquots of important stocks.
  • WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).
  • Keep a good LAB NOTEBOOK so you know what you did.
  • LABEL TUBES
  • Store GLYCEROLS appropriately
  • If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.

DON'Ts


  • Don’t EVER COMPROMISE SAFETY!
  • Don’t Be too shy to ASK QUESTIONS
  • Don’t Start something without THINKING about what you’re doing
  • Don’t Use a MACHINE without having received training on it.
  • Don’t POUR hazardous or unknown chemicals down the sink.
  • Don’t Leave a door to a cold or warm room OPEN.
  • Don’t IGNORE equipment alarms
  • Don’t USE master stocks other than your own without permission.
  • Don’t Leave your stuff lying around in other people’s areas.
  • Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out).
  • Don’t PLAY MUSIC out loud (not everyone likes your taste).
  • Don’t DATE people you work with.*

* May occasionally result in marriage.


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