DOs and DONTs of Good Lab Citizenship - Revision history

Ricardo Vidal at 20:06, 10 June 2008

Ricardo Vidal — Tue, 10 Jun 2008 20:06:19 GMT

←Older revision		Revision as of 20:06, 10 June 2008
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	===DON'Ts===		===DON'Ts===
-		+	<br />
	* Don’t EVER COMPROMISE SAFETY!		* Don’t EVER COMPROMISE SAFETY!
	* Don’t Be too shy to ASK QUESTIONS.		* Don’t Be too shy to ASK QUESTIONS.

Felix Moser at 18:43, 9 June 2008

Felix Moser — Mon, 09 Jun 2008 18:43:57 GMT

←Older revision		Revision as of 18:43, 9 June 2008
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	[[image:caution-32x32.png\|left]]		[[image:caution-32x32.png\|left]]
	===DON'Ts===		===DON'Ts===
		+
	* Don’t EVER COMPROMISE SAFETY!		* Don’t EVER COMPROMISE SAFETY!
	* Don’t Be too shy to ASK QUESTIONS.		* Don’t Be too shy to ASK QUESTIONS.

Felix Moser at 18:43, 9 June 2008

Felix Moser — Mon, 09 Jun 2008 18:43:29 GMT

←Older revision		Revision as of 18:43, 9 June 2008
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	[[image:caution-32x32.png\|left]]		[[image:caution-32x32.png\|left]]
	===DON'Ts===		===DON'Ts===
-	~~<br />~~
	* Don’t EVER COMPROMISE SAFETY!		* Don’t EVER COMPROMISE SAFETY!
-	* Don’t Be too shy to ASK QUESTIONS	+	* Don’t Be too shy to ASK QUESTIONS.
	* Don’t Start something without THINKING about what you’re doing		* Don’t Start something without THINKING about what you’re doing
-	* Don’t Use a MACHINE without having received training on it.	+	* Don’t Use a MACHINE without having received training on it.
-	* Don’t POUR hazardous or unknown chemicals down the sink.	+	* Don’t POUR hazardous or unknown chemicals down the sink. What can be poured down the sink is written on a sheet next to the sink.
-	* Don’t ~~Leave~~ a door to a cold or warm room OPEN.	+	* Don’t pour MOLTEN AGAR down the sink as this clog the pipes below the sink.
		+	* Don’t make a MESS of communal space such as refrigerators
		+	* Don’t put SHARPS in the Biowaste, because they’ll make holes in the plastic, causing molten agar to leak through when autoclaving
		+	* Don’t autoclave in the “kitchen”, during the kitchen working hours (~6am-2pm). Do not leave autoclaved material in the kitchen autoclaved overnight.
		+	* Don’t leave a door to a cold or warm room OPEN.
	* Don’t IGNORE equipment alarms		* Don’t IGNORE equipment alarms
-	* Don’t USE master stocks other than your own without permission.	+	* Don’t USE master stocks other than your own without permission, especially not lab ANTIBIOTICS.
-	* Don’t ~~Leave~~ your stuff lying around in other people’s areas.	+	* Do not hold on to FLASKS or bottles for long after your’re done with them. Instead, when you are done with flasks/bottles, BLEACH them (if you used them to grow cultures) and put them in the metal tray near the sink so they can go back into circulation.
		+	* Don’t just DUMP your biohazard waste into other people’s waste bins and then not clean it up.
		+	* Don’t leave your stuff lying around in other people’s areas.
	* Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out).		* Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out).
-	* Don’t PLAY ~~[http://www.google.com/musicl?lid=pNBLe5O8LxK&aid=qctBmzNNw9O~~ MUSIC] out loud (not everyone likes your taste).	+	* Don’t PLAY MUSIC out loud (not everyone likes your taste).
	* Don’t DATE people you work with.*		* Don’t DATE people you work with.*

Felix Moser at 18:42, 9 June 2008

Felix Moser — Mon, 09 Jun 2008 18:42:14 GMT

←Older revision		Revision as of 18:42, 9 June 2008
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	<br />		<br />
	* Stay SAFE!		* Stay SAFE!
-	∑ Be CONSIDERATE of others (their stuff, needs, space, etc)	+	* Be CONSIDERATE of others (their stuff, needs, space, etc)
-	∑ Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.	+	* Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.
-	∑ THINK before you do.	+	* THINK before you do.
-	∑ CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.	+	* CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.
-	∑ Maintain a CLEAN workspace.	+	* Maintain a CLEAN workspace.
-	∑ REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)	+	* REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)
-	∑ If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.	+	* If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.
-	∑ Maintain STERILE technique.	+	* Maintain STERILE technique.
-	∑ ASK someone before you move their stuff.	+	* ASK someone before you move their stuff.
-	∑ Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.	+	* Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.
-	∑ BALANCE the centrifuges.	+	* BALANCE the centrifuges.
-	∑ ASK SOMEONE how to use a piece of equipment if you haven’t used it before.	+	* ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
-	∑ If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.	+	* If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.
-	∑ CLOSE refrigerator doors!!	+	* CLOSE refrigerator doors!!
-	∑ Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.	+	* Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
-	∑ READ PROTOCOLS before you attempt them.	+	* READ PROTOCOLS before you attempt them.
-	∑ Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE	+	* Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE
-	∑ CHANGE TIPS between aliquots of important stocks.	+	* CHANGE TIPS between aliquots of important stocks.
-	∑ WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).	+	* WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).
-	∑ Keep a good LAB NOTEBOOK so you know what you did.	+	* Keep a good LAB NOTEBOOK so you know what you did.
-	∑ LABEL TUBES	+	* LABEL TUBES
-	∑ Store GLYCEROLS appropriately	+	* Store GLYCEROLS appropriately
-	∑ If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.	+	* If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.

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Felix Moser at 18:41, 9 June 2008

Felix Moser — Mon, 09 Jun 2008 18:41:23 GMT

←Older revision		Revision as of 18:41, 9 June 2008
Line 6:		Line 6:
	<br />		<br />
	* Stay SAFE!		* Stay SAFE!
-	* Be CONSIDERATE of others (their stuff, needs, space, etc)	+	∑ Be CONSIDERATE of others (their stuff, needs, space, etc)
-	* Always ASK SOMEONE if you’re not sure about something.	+	∑ Always ASK SOMEONE if you’re not sure about something. First ask the iGEM graduate advisors before asking other members of the Endy lab.
-	* THINK before you do.	+	∑ THINK before you do.
-	* CLEAN UP after yourself.	+	∑ CLEAN UP after yourself, both on a day-to-day basis, and once the competition is over. If you’ve caused a spill in a centrifuge, clean it up.
-	* Maintain a CLEAN workspace.	+	∑ Maintain a CLEAN workspace.
-	* Maintain STERILE technique.	+	∑ REFILL the stocks of pipette tip boxes above the PCR machines if you are taking the penultimate or the last box of a kind (e.g. 1000ul tips)
-	* ASK someone before you move their stuff.	+	∑ If we’re out of anything (e.g. gloves, cuvettes), ASK your graduate leaders on how to reorder.
-	* BALANCE the ~~[[Knight:5810R_centrifuge\|~~centrifuges]].	+	∑ Maintain STERILE technique.
-	* ASK SOMEONE how to use a piece of equipment if you haven’t used it before.	+	∑ ASK someone before you move their stuff.
-	* If you use the ~~rotator~~ in the 37C ~~room~~, remember to TURN IT BACK ON	+	∑ Before putting a container into the metal tray for cleaning/sterilizing, REMOVE any labels, BLEACH (if biological material) and RINSE the container.
-	* CLOSE refrigerator doors!!	+	∑ BALANCE the centrifuges.
-	* Use YOUR OWN ~~[[Endy:Making_1%25_Agarose_stock\|~~master stocks]] of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.	+	∑ ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
-	* READ ~~[[Protocols\|~~PROTOCOLS]] before you attempt them.	+	∑ If you use the roller drums or shakers in the 30C or 37C rooms, remember to TURN THEM BACK ON.
-	* Keep any proteins ( ~~[[Materials\|~~endonucleases]], ~~[[DNA_ligation\|~~ligase]], ~~[[Smolke:Taq_Buffer\|~~Taq supermix]]) always ON ICE	+	∑ CLOSE refrigerator doors!!
-	* CHANGE TIPS between aliquots of important stocks.	+	∑ Use YOUR OWN master stocks of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
-	* WEAR GLOVES when working w/ dangerous/sensitive chemicals (~~[[Phenol\|~~phenol]], RNA, etc).	+	∑ READ PROTOCOLS before you attempt them.
-	* Keep a good ~~[[Lab_Notebook\|~~LAB NOTEBOOK]] so you know what you did.	+	∑ Keep any proteins (endonucleases, ligase, Taq supermix) always ON ICE
-	* LABEL TUBES	+	∑ CHANGE TIPS between aliquots of important stocks.
-	* Store ~~[[Endy:Screening_plasmid/v2.0/Glycerol_box\|~~GLYCEROLS]] appropriately	+	∑ WEAR GLOVES when working w/ dangerous/sensitive chemicals (phenol, RNA, etc).
-	* If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents.	+	∑ Keep a good LAB NOTEBOOK so you know what you did.
		+	∑ LABEL TUBES
		+	∑ Store GLYCEROLS appropriately
		+	∑ If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents, but you’ll get in trouble for not letting people know.
		+
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Ricardo Vidal: New page: {| style="border: 1px solid #CCCCCC;" cellpadding="5" width="100%" cellspacing="2" |- |style="background-color:#DBFFCC;" width="50%" valign="top"| left ===DO's==...

Ricardo Vidal — Mon, 09 Jun 2008 18:27:05 GMT

New page: {| style="border: 1px solid #CCCCCC;" cellpadding="5" width="100%" cellspacing="2" |- |style="background-color:#DBFFCC;" width="50%" valign="top"| left ===DO's==...

New page

{| style="border: 1px solid #CCCCCC;" cellpadding="5" width="100%" cellspacing="2"
|-
|style="background-color:#DBFFCC;" width="50%" valign="top"|
[[image:accept-32x32.png|left]]
===DO's===
<br />
* Stay SAFE!
* Be CONSIDERATE of others (their stuff, needs, space, etc)
* Always ASK SOMEONE if you’re not sure about something.
* THINK before you do.
* CLEAN UP after yourself.
* Maintain a CLEAN workspace.
* Maintain STERILE technique.
* ASK someone before you move their stuff.
* BALANCE the [[Knight:5810R_centrifuge|centrifuges]].
* ASK SOMEONE how to use a piece of equipment if you haven’t used it before.
* If you use the rotator in the 37C room, remember to TURN IT BACK ON
* CLOSE refrigerator doors!!
* Use YOUR OWN [[Endy:Making_1%25_Agarose_stock|master stocks]] of chemicals and supplies; we’ll tell you which other supplies you’re allowed to use.
* READ [[Protocols|PROTOCOLS]] before you attempt them.
* Keep any proteins ( [[Materials|endonucleases]], [[DNA_ligation|ligase]], [[Smolke:Taq_Buffer|Taq supermix]]) always ON ICE
* CHANGE TIPS between aliquots of important stocks.
* WEAR GLOVES when working w/ dangerous/sensitive chemicals ([[Phenol|phenol]], RNA, etc).
* Keep a good [[Lab_Notebook|LAB NOTEBOOK]] so you know what you did.
* LABEL TUBES
* Store [[Endy:Screening_plasmid/v2.0/Glycerol_box|GLYCEROLS]] appropriately
* If you did something wrong, CONFESS and tell someone. You won’t get in trouble for accidents.
|style="background-color:#FFCCCC;" valign="top" width="50%"|
[[image:caution-32x32.png|left]]
===DON'Ts===
<br />
* Don’t EVER COMPROMISE SAFETY!
* Don’t Be too shy to ASK QUESTIONS
* Don’t Start something without THINKING about what you’re doing
* Don’t Use a MACHINE without having received training on it.
* Don’t POUR hazardous or unknown chemicals down the sink.
* Don’t Leave a door to a cold or warm room OPEN.
* Don’t IGNORE equipment alarms
* Don’t USE master stocks other than your own without permission.
* Don’t Leave your stuff lying around in other people’s areas.
* Don’t EAT/DRINK in lab (go to the tea room to eat lunch and hang out).
* Don’t PLAY [http://www.google.com/musicl?lid=pNBLe5O8LxK&aid=qctBmzNNw9O MUSIC] out loud (not everyone likes your taste).
* Don’t DATE people you work with.*

<nowiki>*</nowiki> ''May occasionally result in marriage.''
|}

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