Dahlquist:GenePix Pro Software Protocol: Difference between revisions
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(→Using GenePix Pro: pasted in from Olivia's Adjusting Threshold Value protocol) |
(→Generating an Array Quality Report: removed word "value") |
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* Select Array Quality Control | * Select Array Quality Control | ||
* Adjust Vital Statistic Thresholds values to: | * Adjust Vital Statistic Thresholds values to: | ||
Quality Control Limits | Quality Control Limits | ||
Median signal to background > 2.5 | Median signal to background > 2.5 | ||
Mean of Median background < 500 | Mean of Median background < 500 | ||
Revision as of 20:45, 12 April 2010
Using GenePix Pro
Generating an Array Quality Report
- Launch GenePix Pro (select Analysis Only)
- On the right hand side of the screen, select the File icon
- File > Open Results
- Select the desired file
- Select Report tab at the top of the screen
- On the left side of the screen, under Navigate, select the back arrow or home icon
- Select Array Quality Control
- Adjust Vital Statistic Thresholds values to:
Quality Control Limits Median signal to background > 2.5 Mean of Median background < 500 Median Signal to Noise > 4 Median % >B + 1 StdDev > 90 Feature variation < 0.5 Background Variation < 1.2 Features with Saturated pixels < 3.3% Not Found < 18% Bad Features < 7%
- Select Start
- Select Show Printable Version
- File > Print
- Under Select Printer > Select Adobe PDF
- Select Print
- Save on the Desktop
- Name the file: Chip #_Year-Month-Date
- The PDF file will either open on its own or you need to open it from the file you saved it to
- Print the first two pages of the results
