Dahlquist:Protocols

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(Wet Lab Protocols: added link to primer file)
(Wet Lab Protocols: added link to SGD)
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* [[Media:DahlquistLab_ColonyPCRProtocol_YeastDeletionStrains.doc | Yeast Colony PCR]]
* [[Media:DahlquistLab_ColonyPCRProtocol_YeastDeletionStrains.doc | Yeast Colony PCR]]
** For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
** For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
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** To download the file of primer sequences and product sizes, right click on [http://www-sequence.stanford.edu/group/yeast_deletion_project/Deletion_primers_PCR_sizes.txt this link] and choose "Save target as" or "Save link as".  The file is a tab-delimited text file that then can be opened in Excel.
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** To download the file of primer sequences and product sizes, right click on [http://www-sequence.stanford.edu/group/yeast_deletion_project/Deletion_primers_PCR_sizes.txt this link] and choose "Save target as" or "Save link as".  The file is a tab-delimited text file that then can be opened in Excel.  The file uses the systematic name (ORF ID) for the genes which can be looked up at [http://yeastgenome.org SGD].
* [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]]
* [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]]
* [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]]
* [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]]

Revision as of 18:24, 10 June 2010

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Real-time PCR

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