Dahlquist:Protocols

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(added GenePix Pro manual)
(Wet Lab Protocols: link to article on yeast genomic DNA purification)
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** For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
** For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
** To download the file of primer sequences and product sizes, right click on [http://www-sequence.stanford.edu/group/yeast_deletion_project/Deletion_primers_PCR_sizes.txt this link] and choose "Save target as" or "Save link as".  The file is a tab-delimited text file that then can be opened in Excel.  The file uses the systematic name (ORF ID) for the genes which can be looked up at [http://yeastgenome.org SGD].
** To download the file of primer sequences and product sizes, right click on [http://www-sequence.stanford.edu/group/yeast_deletion_project/Deletion_primers_PCR_sizes.txt this link] and choose "Save target as" or "Save link as".  The file is a tab-delimited text file that then can be opened in Excel.  The file uses the systematic name (ORF ID) for the genes which can be looked up at [http://yeastgenome.org SGD].
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** [http://www.biotechniques.com/multimedia/archive/00160/BTN5005-BM-Kristjuh_160476a.pdf Protocol for quick purification of genomic DNA from yeast for PCR (Looke et al. 2011, ''Biotechniques'' 50(5): 325–328); haven't tried this yet, just bookmarking it here.]
* [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]]
* [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]]
* [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]]
* [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]]

Revision as of 14:01, 6 June 2011

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