Dahlquist:Protocols: Difference between revisions
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(→Wet Lab Protocols: added link to sodium phosphate buffer recipe) |
(→Wet Lab Protocols: link to oligo extinction coefficient calculator) |
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* [[Dahlquist:Kodak Gel Logic 100 | Kodak Gel Logic 100]] | * [[Dahlquist:Kodak Gel Logic 100 | Kodak Gel Logic 100]] | ||
* [https://www.msu.edu/~eisthen/lab/methods/anatomy/recipes/PB.html Sodium Phosphate Buffer Recipe] | * [https://www.msu.edu/~eisthen/lab/methods/anatomy/recipes/PB.html Sodium Phosphate Buffer Recipe] | ||
* [http://www.ambion.com/techlib/misc/oligo_calculator.html Oligo Extinction Coefficient Calculator] | |||
=== Real-time PCR === | === Real-time PCR === | ||
Revision as of 13:50, 18 September 2011
Lab Organization
- Laboratory Safety and Citizenship Rules
- Student Contact Information Form
- Guidelines for Paper Laboratory Notebooks
- Check-out Procedure for leaving the lab
- Temperature Log Template (for recording daily temperatures of freezers, incubators, etc.)
Wet Lab Protocols
- DNA Microarray Protocol
- Testing Yeast Strains for Temperature-sensitive Growth on Solid Media
- YEPD Medium
- YEPD Plates
- Autoclave instructions
- Yeast Colony PCR
- For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
- To download the file of primer sequences and product sizes, right click on this link and choose "Save target as" or "Save link as". The file is a tab-delimited text file that then can be opened in Excel. The file uses the systematic name (ORF ID) for the genes which can be looked up at SGD.
- Protocol for quick purification of genomic DNA from yeast for PCR (Looke et al. 2011, Biotechniques 50(5): 325–328); haven't tried this yet, just bookmarking it here.
- Scanning instructions GenePix 4000B
- E. coli Chemically Competent Cell Preparation Protocol
- Kodak Gel Logic 100
- Sodium Phosphate Buffer Recipe
- Oligo Extinction Coefficient Calculator
Real-time PCR
- Bio-Rad CFX Manager Plate Quick Guide
- ABI TaqMan Gene Expression Master Mix Quick Reference Card
- ABI TaqMan Gene Expression Master Mix Protocol
- ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR
- 10 Tips for consistent real-time PCR
- NormFinder software
- geNorm software
Dry Lab Protocols
- Dahlquist:Microarray Data Processing in R
- GenMAPP & MAPPFinder Protocols from the Spring 2010 Bioinformatics Lab course.
- GenePix Pro Software Protocol
- GenePix Pro Manual 6.0
- Edge Software Protocol
- Edge Manual (Note: I posted this because I could no longer find a link to it on the web.)
- Dahlquist:Wiki Checklist
- Changing Drive Letters on Windows XP and 7
- Converting Excel tables into wiki formatting
- Blackboard 9.1 Manual
Reagents
- List of oligonucleotides/primers in Dahlquist Lab
- Yeast Systematic ORF Deletion Project
- pORF yeast primers FTP site
- HP High-Gloss Photo Paper C6565A (for CS poster plotter)
