Dahlquist:Protocols: Difference between revisions
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(→Real-time PCR: another link to ABI documentation) |
(→Real-time PCR: link to RT-PCR tips) |
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* [http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_039284.pdf ABI TaqMan Gene Expression Master Mix Protocol] | * [http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_039284.pdf ABI TaqMan Gene Expression Master Mix Protocol] | ||
* [http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_042380.pdf ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR] | * [http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_042380.pdf ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR] | ||
* [http://bitesizebio.com/2009/01/20/10-tips-for-consistent-real-time-pcr-rtpcr/ 10 Tips for consistent real-time PCR] | |||
== Dry Lab Protocols == | == Dry Lab Protocols == | ||
Revision as of 12:49, 15 October 2009
Lab Organization
- Laboratory Safety and Citizenship Rules
- Student Contact Information Form
- Guidelines for Paper Laboratory Notebooks
- Check-out Procedure for leaving the lab
- Temperature Log Template (for recording daily temperatures of freezers, incubators, etc.)
Wet Lab Protocols
- DNA Microarray Protocol: this protocol is under construction.
- Testing Yeast Strains for Temperature-sensitive Growth on Solid Media
- YEPD Medium
- YEPD Plates
- Autoclave instructions
- Yeast Colony PCR
- For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
- Scanning instructions GenePix 4000B
- E. coli Chemically Competent Cell Preparation Protocol
- Kodak Gel Logic 100
Real-time PCR
- Bio-Rad CFX Manager Plate Quick Guide
- ABI TaqMan Gene Expression Master Mix Quick Reference Card
- ABI TaqMan Gene Expression Master Mix Protocol
- ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR
- 10 Tips for consistent real-time PCR
Dry Lab Protocols
- Edge Software Protocol
- Edge Manual (Note: I posted this because I could no longer find a link to it on the web.)
- Dahlquist:Wiki Checklist
- Changing Drive Letters on Windows XP
