Dahlquist:Protocols

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Lab Organization

• Laboratory Safety and Citizenship Rules
• Student Contact Information Form
• Guidelines for Paper Laboratory Notebooks
• Check-out Procedure for leaving the lab
• Temperature Log Template (for recording daily temperatures of freezers, incubators, etc.)

Wet Lab Protocols

• DNA Microarray Protocol: this protocol is under construction.
• Testing Yeast Strains for Temperature-sensitive Growth on Solid Media
• YEPD Medium
• YEPD Plates
• Autoclave instructions
• Yeast Colony PCR
  • For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
• Scanning instructions GenePix 4000B
• E. coli Chemically Competent Cell Preparation Protocol
  • Dahlquist:Transformation Protocol for E. coli Chemically Competent Cells
• Kodak Gel Logic 100

Real-time PCR

• Bio-Rad CFX Manager Plate Quick Guide
• ABI TaqMan Gene Expression Master Mix Quick Reference Card
• ABI TaqMan Gene Expression Master Mix Protocol
• ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR
• 10 Tips for consistent real-time PCR
• NormFinder software
• geNorm software

Dry Lab Protocols

• GenePix Pro Software Protocol
• Edge Software Protocol
• Edge Manual (Note: I posted this because I could no longer find a link to it on the web.)
• Dahlquist:Wiki Checklist
• Changing Drive Letters on Windows XP
• Converting Excel tables into wiki formatting

Reagents

• List of oligonucleotides/primers in Dahlquist Lab
• Yeast Systematic ORF Deletion Project
  • List of Yeast Deletion Project Primers