Dahlquist:Protocols: Difference between revisions
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* [[Media:DahlquistLab_ColonyPCRProtocol_YeastDeletionStrains.doc | Yeast Colony PCR]] | * [[Media:DahlquistLab_ColonyPCRProtocol_YeastDeletionStrains.doc | Yeast Colony PCR]] | ||
** For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab | ** For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab | ||
** To download the file of primer sequences and product sizes, right click on [http://www-sequence.stanford.edu/group/yeast_deletion_project/Deletion_primers_PCR_sizes.txt this link] and choose "Save target as" or "Save link as". The file is a tab-delimited text file that then can be opened in Excel. | ** To download the file of primer sequences and product sizes, right click on [http://www-sequence.stanford.edu/group/yeast_deletion_project/Deletion_primers_PCR_sizes.txt this link] and choose "Save target as" or "Save link as". The file is a tab-delimited text file that then can be opened in Excel. The file uses the systematic name (ORF ID) for the genes which can be looked up at [http://yeastgenome.org SGD]. | ||
* [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]] | * [[Media:ScannerInstructions.doc | Scanning instructions GenePix 4000B]] | ||
* [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]] | * [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]] | ||
Revision as of 15:24, 10 June 2010
Lab Organization
- Laboratory Safety and Citizenship Rules
- Student Contact Information Form
- Guidelines for Paper Laboratory Notebooks
- Check-out Procedure for leaving the lab
- Temperature Log Template (for recording daily temperatures of freezers, incubators, etc.)
Wet Lab Protocols
- DNA Microarray Protocol: this protocol is under construction.
- Testing Yeast Strains for Temperature-sensitive Growth on Solid Media
- YEPD Medium
- YEPD Plates
- Autoclave instructions
- Yeast Colony PCR
- For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
- To download the file of primer sequences and product sizes, right click on this link and choose "Save target as" or "Save link as". The file is a tab-delimited text file that then can be opened in Excel. The file uses the systematic name (ORF ID) for the genes which can be looked up at SGD.
- Scanning instructions GenePix 4000B
- E. coli Chemically Competent Cell Preparation Protocol
- Kodak Gel Logic 100
Real-time PCR
- Bio-Rad CFX Manager Plate Quick Guide
- ABI TaqMan Gene Expression Master Mix Quick Reference Card
- ABI TaqMan Gene Expression Master Mix Protocol
- ABI Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR
- 10 Tips for consistent real-time PCR
- NormFinder software
- geNorm software
Dry Lab Protocols
- GenePix Pro Software Protocol
- Edge Software Protocol
- Edge Manual (Note: I posted this because I could no longer find a link to it on the web.)
- Dahlquist:Wiki Checklist
- Changing Drive Letters on Windows XP
- Converting Excel tables into wiki formatting
