Dahlquist:SURP 2015 Schedule

From OpenWetWare
Jump to navigationJump to search

Home        Research        Protocols        Notebook        People        Publications        Courses        Contact       


SURP 2015 Schedule

Week Date Wet Lab GRNsight GRNmap Meetings & Events
1 Mon

May 18

AM: How to wiki, PowerPoint overview

PM: Within- and between-array normalization, one-strain ANOVA

Tues

May 19

AM: Modified T test

PM: Between-strain ANOVA, clustering with stem, preparation of GenMAPP input sheet, "Deception at Duke" video, "Baggerly: Forensic Bioinformatics" video, research ethics discussion

  • SURP BBQ, 12:00-1:00 PM, Bird's Nest
Wed

May 20

  • Decide which strain to label and hybridize to microarrays: Δino2, Δopi1, or Δyap1
  • Decide which strains to test for growth impairment in cold: Δarg80, Δrsf2, Δrtg3, Δtbf1, Δyhp1, Δyox1, Δphd1, Δnrg1 or other
2 Tues

May 26

AM: F1 cDNA synthesis

PM: F1 cDNA purification, aRNA synthesis

Wed

May 27

  • Joint group meeting, 12:00-2:00 PM, S202
Thurs

May 28

AM: F1 aRNA purification, quantification

PM: F1 aRNA gel

  • Glenn Johnson-Grau: workshop on searching the literature
  • Nicole's last day
3 Mon

June 1

AM: dry F1 aRNA, F1 cDNA synthesis

PM: F1 label & hybe

Tues

June 2

AM: F1 wash & scan

PM: F2 cDNA purification, aRNA synthesis

Wed

June 3

AM: F2 aRNA purification, quantification

PM: F2 aRNA gel

4 Mon

June 8

AM: dry F2 aRNA, F3 cDNA synthesis

PM: F2 label & hybe

Tues

June 9

AM: F2 wash & scan

PM: F3 cDNa purification, aRNA synthesis

Wed

June 10

AM: F3 aRNA purification, quantification

PM: F3 aRNA gel

  • Joint group meeting, 12:00-2:00 PM, S202
5 Mon

June 15

AM: dry F3 aRNA, F4 cDNA synthesis

PM: F3 label & hybe

(KD at BioQUEST workshop)
Tues

June 16

AM: F3 wash & scan

PM: F4 cDNA purification, aRNA synthesis

(KD at BioQUEST workshop)
Wed

June 17

AM: F4 aRNA purification, quantification

PM: F4 aRNA gel

  • Joint group meeting, 12:00-2:00 PM, S202
    • Brandon & (Grace) presents journal club
  • Natalie's last day
6 Mon

June 22

AM:

PM: F4 label & hybe

Tues

June 23

AM: F4 wash & scan
Wed

June 24

Lab check-out

Lab check-out

Lab check-out


Strain Voting

Δino2

Δopi1

Δyap1

Δarg80

Δrsf2

Δrtg3

Δtbf1

Δyhp1

Δyox1

Δphd1

Δnrg1