Dahlquist:Yeast Transcription Factors

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Dahlquist Lab Yeast Transcription Factor Resources

This page displays a compiled list of yeast transcription factor resources. Included are data on transcription factor target genes and transcription factor regulatory networks in yeast, links to web servers providing easy-to-use TF searches and other potentially helpful resources.

TF Network Data

• Lee 2002
  • Pub med reference = 12399584
• Harbison 2004
  • Pub med reference = 15343339
• MacIsaac 2006
  • Pub med reference = 16522208

//Updated TF network to determine new relationships Used improved algorithms to detect new regulatory relationships in the transcription factor network Fig. 3 Shows that the effect on Cin5 was to gain approximately 10 new interactions and lose approximately two as compared to the older model. A figure of the new network was downloaded. PDF format

Supplementary Data Web Site

Supplementary Data in zipped .xls file

• Workman 2006
  • Pub med reference = 16709784

//TF-target interactions in DNA damage response Measured binding locations(ChIP-chip) of damage-related transcription factors (including Cin5) after exposure to MMS. Validated by checking against binding in knock-out strains for TFs. Downloaded the MMS and Untreated ChIP datasets to be filtered for Cin5p targets, Excel spreadsheet of p values for binding.

• Hu 2007
  • Pub med reference = 17417638

//TF network discovered through systematic deletions Performed microarray analysis of knockout strains for many transcription factors and compared expression profiles to determine regulatory relationships. Allows distinguishing between activated and repressed transcription factor targets Downloaded the filtered and unfiltered network in X-score format. Gene-gene-value triples.

• Jothi 2009
  • Pub med reference = 19690563
• Balaji 2006
  • Pub med reference = 16762362

//Full text from ScienceDirect

• Bar-Joseph 2003
  • Pub med reference = 14555958

//Abstract and Supplemental Data

• Ernst 2007
  • Pub med reference = 17224918

//Cold Shock Temporal Network Data Used new algoithm to construct temporal networks of yeast's response to various stresses, including cold shock. For cold shock data, the paper analyzes data from the Gasch, et al. (2000) paper. Supplementary data has cold shock temporal response, but does not show any interactions between transcription factors and targets. (Note: the Gasch et al. paper does not have a true "cold shock" response. They only measure the effect of a temperature downshift from 37°C to 25°C. Cold shock is defined in the range of 10°C to 18°C

• Zhu 2009
  • Pub med reference = 19158363
• Kim 2006
  • Pub med reference = 16551355

Web Servers

• MYBS Mining Yeast Binding Sites
  • The search regulatory associations function allows the user to input a list of genes of interest and search for candidate transcription factors that regulate the list of genes. Allows filtering according to multiple criteria.
  • From Tsai, HK, et al. MYBS: A comprehensive web server for mining transcription factor binding sites in yeast. Nucleic Acids Research 2007. PubMed
• TFSVM Transcription Factor Support Vector Machine
  • Allows user to input either a single transcription factor to get a list of regulated target genes or a single gene to get a list of transcription factors that regulate that gene. Can also filter according to a threshold value
  • Downloaded the list of Cin5p targets with a threshold value >0.997.
  • From Holloway, DT, et al. Classifying transcription factor targets and discovering relevant biological features. Biology Direct 2008. 3:22. PubMed
• YEASTRACT Yeast Search for Transcriptional Regulators and Consensus Tracking
  • Multiple tools are provided on this server. Those of interest include a search for regulatory relationships either by transcription factor or by target gene. Applies a different algorithm to find new regulatory relationships in previously published data from Lee, Harbison, Tan and Workman
  • Downloaded list of documented Cin5p targets.
  • From Monteiro, PT, et al. YEASTRACT-DISCOVERER: new tools to improve analysis of transcriptional regulatory associations in Saccharomyces cerevisiae. Nucleic Acids Research 2008. PubMed
• ChIPCodis Mining of Regulatory Transcription Factor Data in Yeast
  • Allows the user to input a list of genes and provides list of candidate transcription factors that regulate. References either Harbison or MacIsaac data.
  • From Abascal, F et al. ChIPCodis: mining complex regulatory systems in yeast by concurrent enrichment analysis of chip-on-chip data. Bioinformatics 2008. PubMed
• List of Yeast Transcription Factors (don't know when this web page was updated)
• Jaspar TF binding site motif database