Dandekar & Chandler:AHL Extraction

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===Principle===
===Principle===
 +
Assay for acyl-HSL detection using E. coli reporters containing pQF50- (QS-controlled lacZ fusion, Ap) and pJN105- (arabinose inducible R gene, Gm) derived plasmids.
===Materials Needed===
===Materials Needed===

Revision as of 18:28, 18 October 2013

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Contents

N-Acyl homoserine lactone (AHL) Extraction

Principle

Assay for acyl-HSL detection using E. coli reporters containing pQF50- (QS-controlled lacZ fusion, Ap) and pJN105- (arabinose inducible R gene, Gm) derived plasmids.

Materials Needed

  • Spectrophotometer capable of reading 520 nm
  • Vortex
  • 10mL test tube
  • Sterile Cuvets
  • 5ml glass pipet
  • Nanopure H20
  • 0.2m HCl
  • Chloroform

Protocol

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