Dandekar & Chandler:AHL Extraction: Difference between revisions
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===Principle=== | ===Principle=== | ||
Assay for acyl-HSL detection using E. coli reporters containing pQF50- (QS-controlled lacZ fusion, Ap) and pJN105- (arabinose inducible R gene, Gm) derived plasmids. | |||
===Materials Needed=== | ===Materials Needed=== | ||
Revision as of 15:28, 18 October 2013
N-Acyl homoserine lactone (AHL) Extraction
Principle
Assay for acyl-HSL detection using E. coli reporters containing pQF50- (QS-controlled lacZ fusion, Ap) and pJN105- (arabinose inducible R gene, Gm) derived plasmids.
Materials Needed
- Spectrophotometer capable of reading 520 nm
- Vortex
- 10mL test tube
- Sterile Cuvets
- 5ml glass pipet
- Nanopure H20
- 0.2m HCl
- Chloroform
