Dandekar & Chandler:AHL Extraction

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N-Acyl homoserine lactone (AHL) Extraction

Principle

Assay for acyl-HSL detection using E. coli reporters containing pQF50- (QS-controlled lacZ fusion, Ap) and pJN105- (arabinose inducible R gene, Gm) derived plasmids.

Materials Needed

• Spectrophotometer capable of reading 520 nm
• Vortex
• 10mL test tube
• Sterile Cuvets
• 5ml glass pipet
• Nanopure H20
• 0.2m HCl
• Chloroform