Dandekar & Chandler:M9 Salts: Difference between revisions
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Adjust pH to 7.4, autoclave | Adjust pH to 7.4, autoclave | ||
Before use at 1X add: | |||
* 2.0 mL of sterile filtered 1M MgSO4 | |||
*0.1 mL of sterile filtered 1M CaCl2 | |||
Latest revision as of 09:54, 13 September 2016
10X M9 Salts (per Liter):
- Na2HPO4 60 g
- KH2PO4 30 g
- NaCl 5 g
- NH4Cl 10 g
Adjust pH to 7.4, autoclave
Before use at 1X add:
- 2.0 mL of sterile filtered 1M MgSO4
- 0.1 mL of sterile filtered 1M CaCl2
