Dandekar & Chandler:MIC protocol

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Nicole Smalley (Talk | contribs)
(New page: ==MIC Protocol== '''Materials needed (all must be sterile):''' :• Mueller Hinton Broth ::- From Parsek lab: MHB broth BBL II, Cation Adjusted (11g/500mL) :• Tryptic Soy Broth :• Pho...)
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Revision as of 16:39, 22 August 2013

MIC Protocol

Materials needed (all must be sterile):

• Mueller Hinton Broth
- From Parsek lab: MHB broth BBL II, Cation Adjusted (11g/500mL)
• Tryptic Soy Broth
• Phosphate Buffered Saline
• Costar 3370: 96 well assay plates
• Breathe-Easy 9123-6100 gas permeable sealing membrane


Day 1:

1. Streak relevant strains onto LB plate and incubate at 37C

a. TT Box 1:
i. WT 3610 - H4
ii. 1.19 (E236A) - B1
iii. 3.3 (235G) - B7
iv. 3.4 (E236Q) - B8
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