Dandekar & Chandler:MIC protocol
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MIC Protocol
Materials needed (all must be sterile):
- • Mueller Hinton Broth
- - From Parsek lab: MHB broth BBL II, Cation Adjusted (11g/500mL)
- • Tryptic Soy Broth
- • Phosphate Buffered Saline
- • Costar 3370: 96 well assay plates
- • Breathe-Easy 9123-6100 gas permeable sealing membrane
Day 1:
1. Streak relevant strains onto LB plate and incubate at 37C
- a. TT Box 1:
- i. WT 3610 - H4
- ii. 1.19 (E236A) - B1
- iii. 3.3 (235G) - B7
- iv. 3.4 (E236Q) - B8
