Davidson:Protocols: Difference between revisions
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{{Davidson Top}} | {{Davidson Top}} | ||
=Wet Lab Protocols= | |||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents] | ||
# [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly] | # [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/ORIs.html | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/ORIs.html Compatibility of Plasmids] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg<sup>2+</sup> concentration] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg<sup>2+</sup> concentration] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/Clean_Concentrate.html Clean and Concentrate DNA (after PCR, before digestion)] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Clean_Concentrate.html Clean and Concentrate DNA (after PCR, before digestion)] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs] | ||
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# [http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Heat Shock Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version of Heat Shock] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Heat Shock Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version of Heat Shock] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/Zippy_Transformation.html Zippy Transformation] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Zippy_Transformation.html Zippy Transformation] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/ColonyPCR_Screening.html Colony PCR to Screen for Successful Ligations] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/ColonyPCR_Screening.html Colony PCR to Screen for Successful Ligations] | ||
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/Tranformation_list.html Choices for Transformation: Heat Shock vs. Zyppy] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/Tranformation_list.html Choices for Transformation: Heat Shock vs. Zyppy] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | # [http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | ||
#[[Davidson:Colony_PCR | Colony PCR]] | # [[Davidson:Colony_PCR | Colony PCR]] | ||
Latest revision as of 14:07, 13 November 2007
