De la Cruz:Publications

Group papers in the last 5 years

Tato I, Matilla I, Arechaga I, Zunzunegui S, de la Cruz F, Cabezon E.
The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA: implications for a common assembly mechanism of DNA translocating motors
J Biol Chem. 2007 Aug 31;282(35):25569-76. Epub 2007 Jun 27. 

Gonzalez-Perez B, Lucas M, Cooke LA, Vyle JS, de la Cruz F, Moncalián G. (2007)

Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides. EMBO J. 2007 Aug 22;26(16):3847-57. Epub 2007 Jul 26.

Sanchez-Magraner L, Viguera AR, Garcia-Pacios M, Garcillan MP, Arrondo JL, de la Cruz F, Goni FM, Ostolaza H (2007).

The calcium-binding C-terminal domain of Escherichia coli alpha -haemolysin is a major determinant in the surface-active properties of the protein. J Biol Chem. Feb 26;

Garcillán-Barcia MP, Jurado P, González-Pérez B, Moncalián G, Fernández LA, de la Cruz F (2007).

Conjugative transfer can be inhibited by blocking relaxase activity within recipient cells with intrabodies Mol Microbiol 63(2):404-16.

Moncalián G, Cardenes N, Deribe YL, Spinola-Amilibia M, Dikic I, Bravo J (2006). 

Atypical polyproline recognition by the CMS N-terminal Src homology 3 domain. J Biol Chem. 281(50):38845-53.

César CE, Machón C, de la Cruz F, Llosa M (2006).

A new domain of conjugative relaxase TrwC responsible for efficient oriT-specific recombination on minimal target sequences Mol Microbiol 62(4):984-996

Fernández-López R, Garcillán-Barcia MP, Revilla C, Lázaro M, Vielva L, de la Cruz F (2006). Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution FEMS Microbiol Rev 30(6):942-966..

Yim G, de la Cruz F, Spiegelman GB, Davies J (2006). Transcription modulation of S. typhimurium promoters by sub-MIC levels of rifampicin J Bacteriol 188(22):7988-7991.

Perez-Mendoza D, Lucas M, Munoz S, Herrera-Cervera JA, Olivares J, de la Cruz F, Sanjuan J (2006). The relaxase of the Rhizobium etli symbiotic plasmid shows nic site cis-acting preference. J Bacteriol 188(21):7488-7499.

Hormaeche I, Segura RL, Vecino AJ, Goni FM, de la Cruz F, Alkorta I (2006). The transmembrane domain provides nucleotide binding specificity to the bacterial conjugation protein TrwB. FEBS Lett 580(13):3075-82.

Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Pérez-Luque R, Coll M, de la Cruz F (2006). Unveiling the Molecular Mechanism of a Conjugative Relaxase: The Structure of TrwC Complexed with a 27-mer DNA Comprising the Recognition Hairpin and the Cleavage Site J Mol Biol 358(3):857-869.

Cabezón E, de la Cruz F (2006). TrwB: An F1-ATPase-like molecular motor involved in DNA transport during bacterial conjugation Res Microbiol 157:299–305.

de la Cruz F, Davies J (2005). Industrial revolution and microbial evolution”. En: “The influence of cooperative bacteria on animal host biology (Eds. M.J. McFall-Ngai, B. Henderson, E.G. Ruby), pp. 73-82. Advances in Molecular and Cellular Microbiology, vol. 10., Cambridge University Press, Cambridge USA.

Draper O, César CE, Machón C, de la Cruz F, Llosa M (2005). Site-specific recombinase and integrase activities of a conjugative relaxase in the recipient cell. Proc Natl Acad Sci USA 102:16385-16390.

de Paz HD, Sangari FJ, Bolland S, García-Lobo JM, Dehio C, de la Cruz F, Llosa M (2005) Functional interactions between Type IV secretion systems involved in DNA transfer and virulence Microbiology 151:3505-3516

Fernández-López R, Machón C, Longshaw CM, Martin S, Molin S, Zechner EL, Espinosa M, Lanka E, de la Cruz F (2005) Unsaturated fatty acids are inhibitors of bacterial conjugation Microbiology 151:3517-3526.

Camacho EM, Serna A, Madrid C, Marqués S, Fernández R, de la Cruz F, Juárez A, Casadesús J (2005) Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica J Bacteriol.187:5691-5699

Tato I, Zunzunegui S, de la Cruz F, Cabezón E (2005) TrwB, the coupling protein involved in DNA transport during bacterial conjugation, is a DNA-dependent ATPase Proc Natl Acad Sci USA 102:8156-61.

Llosa M, de la Cruz F (2005) Bacterial conjugation: a potential tool for genomic engineering Res Microbiol. 156:1-6. Moncalián G, de la Cruz F (2004) DNA binding properties of protein TrwA, a possible structural variant of the Arc repressor superfamily Biochim Biophys Acta 1701:15-23.

Gomis-Ruth FX, Sola M, de la Cruz F, Coll M. (2004) Coupling factors in macromolecular type-IV secretion machineries. Curr Pharm Des.10:1551-65.

Francia MV, Varsaki A, Garcillan-Barcia MP, Latorre A, Drainas C, de la Cruz F. (2004). A classification scheme for mobilization regions of bacterial plasmids. FEMS Microbiol Rev. 28(1):79-100.

Hormaeche I, Iloro I, Arrondo JL, Goni FM, de la Cruz F, Alkorta I. (2004). Role of the transmembrane domain in the stability of TrwB, an integral protein involved in bacterial conjugation. J Biol Chem. 279(12):10955-61. .

Guasch A, Lucas M, Moncalian G, Cabezas M, Perez-Luque R, Gomis-Ruth FX, de la Cruz F, Coll M. (2003) Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC. Nat Struct Biol. 10(12):1002-10.

Seubert A, Hiestand R, de la Cruz F, Dehio C. (2003) A bacterial conjugation machinery recruited for pathogenesis Mol Microbiol. 49(5):1253-66.

Llosa M, Zunzunegui S, de la Cruz F. (2003) Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes Proc Natl Acad Sci U S A. 100(18):10465-70

Varsaki A, Lucas M, Afendra AS, Drainas C, de la Cruz F. (2003) Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilization Mol Microbiol. 48(2):481-93

Arrondo JL, Echabe I, Iloro I, Hernando MA, de la Cruz F, Goñi FM. (2003). A bacterial TrwC relaxase domain contains a thermally stable alpha-helical core. J Bacteriol. 185(14):4226-32.

Selected papers between 2003- 2000

Llosa M., F.X. Gomis-Rüth, M. Coll y F. de la Cruz (2002)

Bacterial conjugation: a two-step mechanism for DNA transport

Mol Microbiol. 153:199-204.

de la Cruz F., J.M. García-Lobo y J. Davies (2002) Antibiotic resistance: on how bacterial populations respond to a simple evolutionary force

 In  “Bacterial Resistance to Antimicrobials:  Mechanisms, Genetics, Medical Practice and Public Health” pp. 19-36 (K. Lewis, A. Salyers, H. Taber, y R. Wax, editors)  Ed. Marcel Dekker

Garcillán-Barcia M.P., I. Bernales, M.V. Mendiola, F. de la Cruz (2001) IS91 rolling-circle transposition

in “Mobile DNA II” (N.L. Craig, R. Craigie, M. Gellert, A. Lambowitz, eds.) ASM Press, Washington.

Núñez B., F. de la Cruz (2001) Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation Mol Microbiol. 39:1088-1099.

Gomis-Ruth F.X., G. Moncalián, R. Pérez Luque, A. González, E. Cabezón, F. de la Cruz, M. Coll (2001)

The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase Nature 409:637-641

Garcillán-Barcia M.P., I. Bernales, M.V. Mendiola, F. de la Cruz (2001) Single-stranded DNA intermediates in IS91 rolling-circle transposition

Mol Microbiol. 39:494-502. 

de la Cruz F., J. Davies (2000) Horizontal gene transfer and the origin of species: lessons from bacteria Trends Microbiol. 8:128-133.

Grandoso G., P. Avila, A. Cayón, M.A. Hernando, M. Llosa, F. de la Cruz (2000) Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation

 J Mol Biol. 295:1163-1172. 

Zechner, E. L., de la Cruz, F., Eisenbrandt, R., Grahn, A. M., Koraimann, G., Lanka, E., Muth, G., Pansegrau, W., Thomas, C. M., Wilkins, B. M., Zatyka, M. (2000).  Conjugative-DNA transfer processes  

in "The Horizontal Gene Pool: Bacterial Plasmids and Gene Spread" (Thomas, C. M., ed.), pp. 87-173. Harwood Academic Publishers, London.