Defining the network of ROP GTPase signalling and its regulation in root hair development.: Difference between revisions
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Woodland Road | Woodland Road | ||
Bristol. UK | Bristol. UK | ||
matt.smallman@bristol.ac.uk | matt.smallman@bristol.ac.uk | ||
[[ | [[http://openwetware.org/wiki/Grierson_Lab_Wiki]] | ||
===Education=== | ===Education=== | ||
* | * 2004-present, PhD, University of Bristol | ||
* | * 2003, MSc, Nottingham Trent | ||
* | * 1999, BSc (Hons), Oxford Brookes University | ||
=== Defining the network of ROP GTPase signalling and its regulation in root hair development === | === Defining the network of ROP GTPase signalling and its regulation in root hair development === | ||
Rho small GTPases are conserved molecular switches in eukaryotic signal transduction involved in a variety of biological processes such as the establishment of cell polarity and actin remodeling. The GTPase switch is closely controlled through regulatory mechanism involving at least two kinds of negative regulators: the Rho Guanine nucleotide Dissociation Inhibitors (GDIs) and Rho GTPase activating proteins (GAPs). Our previous work (Jones et al, 2002) has shown that the over-expression of the Rho small GTPase ROP2 in Arabidopsis produces a root hair phenotype similar to the loss of function scn1 mutants (Parker et al, 2000.) The SCN1 locus (Parker et al, 2000) encodes a Rho-GDI (AtROPGDI1), which in other systems negatively regulate Rho GTPases by sequestering the protein away from the plasma membrane. Affymetrix expression data of the Arabidopsis root hair transcriptome has allowed us to identify several potential candidates involved in the ROP2/GDI1 signalling pathway including a GAP that affects root hair growth. | Rho small GTPases are conserved molecular switches in eukaryotic signal transduction involved in a variety of biological processes such as the establishment of cell polarity and actin remodeling. The GTPase switch is closely controlled through regulatory mechanism involving at least two kinds of negative regulators: the Rho Guanine nucleotide Dissociation Inhibitors (GDIs) and Rho GTPase activating proteins (GAPs). Our previous work (Jones et al, 2002) has shown that the over-expression of the Rho small GTPase ROP2 in Arabidopsis produces a root hair phenotype similar to the loss of function scn1 mutants (Parker et al, 2000.) The SCN1 locus (Parker et al, 2000) encodes a Rho-GDI (AtROPGDI1), which in other systems negatively regulate Rho GTPases by sequestering the protein away from the plasma membrane. Affymetrix expression data of the Arabidopsis root hair transcriptome has allowed us to identify several potential candidates involved in the ROP2/GDI1 signalling pathway including a GAP that affects root hair growth. | ||
Revision as of 06:10, 5 September 2006
Matt Smallman
Contact Info
Matt Smallman School of Biological Sciences University of Bristol Woodland Road Bristol. UK
matt.smallman@bristol.ac.uk
[[1]]
Education
- 2004-present, PhD, University of Bristol
- 2003, MSc, Nottingham Trent
- 1999, BSc (Hons), Oxford Brookes University
Defining the network of ROP GTPase signalling and its regulation in root hair development
Rho small GTPases are conserved molecular switches in eukaryotic signal transduction involved in a variety of biological processes such as the establishment of cell polarity and actin remodeling. The GTPase switch is closely controlled through regulatory mechanism involving at least two kinds of negative regulators: the Rho Guanine nucleotide Dissociation Inhibitors (GDIs) and Rho GTPase activating proteins (GAPs). Our previous work (Jones et al, 2002) has shown that the over-expression of the Rho small GTPase ROP2 in Arabidopsis produces a root hair phenotype similar to the loss of function scn1 mutants (Parker et al, 2000.) The SCN1 locus (Parker et al, 2000) encodes a Rho-GDI (AtROPGDI1), which in other systems negatively regulate Rho GTPases by sequestering the protein away from the plasma membrane. Affymetrix expression data of the Arabidopsis root hair transcriptome has allowed us to identify several potential candidates involved in the ROP2/GDI1 signalling pathway including a GAP that affects root hair growth.
