Dilkes:Research

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(New page: =Notes on Construction= Yup, Beth Toener would be proud. This page is currently under construction. Check back for protocols and updates. ==Submission Procedures and Internal Protocols h...)
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=Notes on Construction=
 
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Yup, Beth Toener would be proud.
 
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This page is currently under construction. Check back for protocols and updates.
 
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==Submission Procedures and Internal Protocols here at the UC Davis!==
 
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# To get [[Davis Sequencing Center Submission| sequence from a few samples]] try [Davis Sequencing Center]
 
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# For more than 30 samples it is more economical to use the Genomics Facility run by Doug Cook and compatriots.
 
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## We do sequencing direct from PCR products via an [[Exo Sap]] treatment prior to submission
 
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## [[CGF Submission]] is relatively straightforward but requires that you set up an account with them.
 
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# There are off campus options including Robert Tran of the Davis Tilling Facility's favorite at [[Rob Tran's Favorite sequencing]]
 
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==Comai Lab Primer Procedures==
 
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# Using the primer database to find your primer
 
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# Ordering primers not in the collection
 
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# Adding new entries to the primer database
 
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==Comai Lab Polyploidy Procedures==
 
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# Fluorescent genotyping
 
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# Aneuploidy detection
 
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# FISH Protocol
 
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# Pollen staining and viability measurements
 
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# Seed phenotyping
 
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# GFP and enhancer trap PCR methods
 
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# GFP imaging
 
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# Sample labeling for two color CGH on Arabidopsis Operon arrays (lifted from the good people at [http://www.fhcrc.org FHCRC array core])
 

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