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| - | =Notes on Construction=
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| - | Yup, Beth Toener would be proud.
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| - | This page is currently under construction. Check back for protocols and updates.
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| - | ==Submission Procedures and Internal Protocols here at the UC Davis!==
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| - | # To get [[Davis Sequencing Center Submission| sequence from a few samples]] try [Davis Sequencing Center]
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| - | # For more than 30 samples it is more economical to use the Genomics Facility run by Doug Cook and compatriots.
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| - | ## We do sequencing direct from PCR products via an [[Exo Sap]] treatment prior to submission
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| - | ## [[CGF Submission]] is relatively straightforward but requires that you set up an account with them.
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| - | # There are off campus options including Robert Tran of the Davis Tilling Facility's favorite at [[Rob Tran's Favorite sequencing]]
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| - | ==Comai Lab Primer Procedures==
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| - | # Using the primer database to find your primer
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| - | # Ordering primers not in the collection
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| - | # Adding new entries to the primer database
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| - | ==Comai Lab Polyploidy Procedures==
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| - | # Fluorescent genotyping
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| - | # Aneuploidy detection
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| - | # FISH Protocol
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| - | # Pollen staining and viability measurements
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| - | # Seed phenotyping
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| - | # GFP and enhancer trap PCR methods
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| - | # GFP imaging
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| - | # Sample labeling for two color CGH on Arabidopsis Operon arrays (lifted from the good people at [http://www.fhcrc.org FHCRC array core])
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Current revision
