Disrupting the biofilm

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search

Joao B. Xavier (Talk | contribs)
(New page: The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain compo...)
Next diff →

Revision as of 15:39, 12 October 2007

The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.

Biofilm disruption solution

  • 1% SDS
  • 5 mM EDTA
  • 100 mM NaCl
  • 50 mM Tris
  • pH 7.4
Personal tools