Disrupting the biofilm

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(New page: The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain compo...)
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== Biofilm disruption solution ==
== Biofilm disruption solution ==
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* 1% SDS
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* 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate]
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* 5 mM EDTA
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* 5 mM [http://en.wikipedia.org/wiki/Edta EDTA]
* 100 mM NaCl
* 100 mM NaCl
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* 50 mM Tris  
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* 50 mM [http://en.wikipedia.org/wiki/Tris Tris]
* pH 7.4
* pH 7.4

Revision as of 14:46, 12 October 2007

The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.

Biofilm disruption solution

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