Disrupting the biofilm: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain compo...) |
No edit summary |
||
| Line 2: | Line 2: | ||
== Biofilm disruption solution == | == Biofilm disruption solution == | ||
* 1% SDS | * 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate] | ||
* 5 mM EDTA | * 5 mM [http://en.wikipedia.org/wiki/Edta EDTA] | ||
* 100 mM NaCl | * 100 mM NaCl | ||
* 50 mM Tris | * 50 mM [http://en.wikipedia.org/wiki/Tris Tris] | ||
* pH 7.4 | * pH 7.4 | ||
Revision as of 12:46, 12 October 2007
The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.
Biofilm disruption solution
- 1% SDS Sodium dodecyl sulfate
- 5 mM EDTA
- 100 mM NaCl
- 50 mM Tris
- pH 7.4
