Disrupting the biofilm

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Current revision (17:29, 15 October 2007) (view source)
 
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== Biofilm disruption solution ==
== Biofilm disruption solution ==
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* 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate]
+
* 1% SDS [http://en.wikipedia.org/wiki/Sodium_dodecyl_sulfate Sodium dodecyl sulfate] (denaturates protein)
* 5 mM [http://en.wikipedia.org/wiki/Edta EDTA]
* 5 mM [http://en.wikipedia.org/wiki/Edta EDTA]
* 100 mM NaCl
* 100 mM NaCl

Current revision

The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.

Biofilm disruption solution

Protocol

  • Grow biofilm in 96-well plate.
  • Extract liquid (measure OD if necessary) and wash twice.
  • Fill with 150 μm disruption solution.
  • Shake for 30 mins.
  • Measure OD.
  • Measure fluorescences.
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