Disrupting the biofilm

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The crystal violet assay is great to quantify biofilm formation quickly in a high-throughput way for single- and multiple-strain biofilms. Nevertheless, we want to look at the strain composition in each biofilm in a high. For this, we need to disrupt the biofilm back into suspended cells so that we can quantify their relative amounts through fluorescence.

Biofilm disruption solution

Protocol

  • Grow biofilm in 96-well plate.
  • Extract liquid (measure OD if necessary) and wash twice.
  • Fill with 150 μm disruption solution.
  • Shake for 30 mins.
  • Measure OD.
  • Measure fluorescences.
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