DpnI - Revision history

Torsten Waldminghaus: /* Use */

Torsten Waldminghaus — Tue, 03 Jul 2012 12:29:37 GMT

Use

←Older revision		Revision as of 12:29, 3 July 2012
Line 11:		Line 11:
	*Heat inactivate by incubating at 80°C for 20 minutes.		*Heat inactivate by incubating at 80°C for 20 minutes.
	*DpnI cleaves '''hemi'''-adenomethylated dam sites 60X more slowly than fully methylated. [http://www.neb.com/nebecomm/products/faqproductR0176.asp#10 NEB FAQ]		*DpnI cleaves '''hemi'''-adenomethylated dam sites 60X more slowly than fully methylated. [http://www.neb.com/nebecomm/products/faqproductR0176.asp#10 NEB FAQ]
-	*Plasmids derived from Dam+ e.coli strains will be a substrate	+	*Plasmids derived from Dam+ ''E.coli'' strains will be a substrate

	===Relevant protocols===		===Relevant protocols===

Roy Ummels: /* Use */

Roy Ummels — Wed, 23 Apr 2008 18:05:29 GMT

Use

←Older revision		Revision as of 18:05, 23 April 2008
Line 11:		Line 11:
	*Heat inactivate by incubating at 80°C for 20 minutes.		*Heat inactivate by incubating at 80°C for 20 minutes.
	*DpnI cleaves '''hemi'''-adenomethylated dam sites 60X more slowly than fully methylated. [http://www.neb.com/nebecomm/products/faqproductR0176.asp#10 NEB FAQ]		*DpnI cleaves '''hemi'''-adenomethylated dam sites 60X more slowly than fully methylated. [http://www.neb.com/nebecomm/products/faqproductR0176.asp#10 NEB FAQ]
		+	*Plasmids derived from Dam+ e.coli strains will be a substrate

	===Relevant protocols===		===Relevant protocols===

Roy Ummels: /* Purpose */

Roy Ummels — Wed, 23 Apr 2008 18:04:16 GMT

Purpose

←Older revision		Revision as of 18:04, 23 April 2008
Line 1:		Line 1:
	==Purpose==		==Purpose==
-	*To digest methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.	+	*To digest (Adeno) methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.

	==Procurement==		==Procurement==

Torsten Waldminghaus: /* Use */

Torsten Waldminghaus — Fri, 28 Mar 2008 15:10:32 GMT

Use

←Older revision		Revision as of 15:10, 28 March 2008
Line 10:		Line 10:
	*Incubate at 37°C.		*Incubate at 37°C.
	*Heat inactivate by incubating at 80°C for 20 minutes.		*Heat inactivate by incubating at 80°C for 20 minutes.
		+	*DpnI cleaves '''hemi'''-adenomethylated dam sites 60X more slowly than fully methylated. [http://www.neb.com/nebecomm/products/faqproductR0176.asp#10 NEB FAQ]

	===Relevant protocols===		===Relevant protocols===

Reshma P. Shetty at 17:05, 27 May 2007

Reshma P. Shetty — Sun, 27 May 2007 17:05:59 GMT

←Older revision		Revision as of 17:05, 27 May 2007
Line 22:		Line 22:
	==Contact==		==Contact==
	*[[Reshma Shetty]]		*[[Reshma Shetty]]
		+
		+	[[Category:Material]] [[Category:Enzyme]] [[Category:Endonuclease]]

Reshma P. Shetty at 17:18, 7 November 2006

Reshma P. Shetty — Tue, 07 Nov 2006 17:18:15 GMT

New page

==Purpose==
*To digest methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.

==Procurement==
*Can be ordered from [http://www.neb.com/ NEB].
*Typically stored at -20°C.

==Use==
*Use in NEBuffer 4. However, can be added directly to PCR sample.
*Incubate at 37°C.
*Heat inactivate by incubating at 80°C for 20 minutes.

===Relevant protocols===
*[[Site-directed mutagenesis]]

==Safety==
*None known.

==References==
*[http://www.neb.com/nebecomm/products/productR0176.asp DpnI from NEB]

==Contact==
*[[Reshma Shetty]]