Drummond:Confocal

1. Turn on mercury lamp. (After 1/2 hour, turn down to 50% power.)
2. Turn on main power.
3. Turn on computer
4. Turn on lasers:
   1. Helium-neon 1
   2. Argon
5. Open LSM510 software

Slide preparation

(cf. cell preparation

• Prepare 15 mins in advance to allow drying
• 15uL droplet
• No. 1 1/2 cover slip
• Push down gently on cover slip to squish out extra liquid and any air bubbles. Cover slip should not float!