Drummond:Lysis

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from [http://www.fhcrc.org/science/labs/gottschling/yeast/yeastip.html?&&printfriendly=yes Yeast Protocols]
from [http://www.fhcrc.org/science/labs/gottschling/yeast/yeastip.html?&&printfriendly=yes Yeast Protocols]
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==Chemical lysis==
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Myristyl sulfobetaine
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3-(N-tetradecyl-N,N-dimethyl-ammonio)-propane-1-sulfonate (SB14) <cite>ZannaJChr07</cite>, available from Sigma as [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/T7763 SB3-14].
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==References==
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<biblio>
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#ZannaJChr07 pmid=16978932
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</biblio>

Revision as of 13:00, 7 July 2007

SUME Buffer (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA) To make 100ml:

   * 1.0ml 1M MOPS, pH6.8 stock solution
   * 1.0g SDS, or 10ml 10% SDS stock solution
   * 48.05g Urea
   * 2.0ml 0.5M EDTA stock solution

This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.

from Yeast Protocols

Chemical lysis

Myristyl sulfobetaine 3-(N-tetradecyl-N,N-dimethyl-ammonio)-propane-1-sulfonate (SB14) [1], available from Sigma as SB3-14.

References

  1. Zanna L and Haeuw JF. . pmid:16978932. PubMed HubMed [ZannaJChr07]
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