Drummond:Lysis
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from [http://www.fhcrc.org/science/labs/gottschling/yeast/yeastip.html?&&printfriendly=yes Yeast Protocols] | from [http://www.fhcrc.org/science/labs/gottschling/yeast/yeastip.html?&&printfriendly=yes Yeast Protocols] | ||
| + | |||
| + | ==Chemical lysis== | ||
| + | Myristyl sulfobetaine | ||
| + | 3-(N-tetradecyl-N,N-dimethyl-ammonio)-propane-1-sulfonate (SB14) <cite>ZannaJChr07</cite>, available from Sigma as [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/T7763 SB3-14]. | ||
| + | |||
| + | ==References== | ||
| + | <biblio> | ||
| + | #ZannaJChr07 pmid=16978932 | ||
| + | </biblio> | ||
Revision as of 14:00, 7 July 2007
SUME Buffer (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA) To make 100ml:
* 1.0ml 1M MOPS, pH6.8 stock solution * 1.0g SDS, or 10ml 10% SDS stock solution * 48.05g Urea * 2.0ml 0.5M EDTA stock solution
This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.
from Yeast Protocols
Chemical lysis
Myristyl sulfobetaine 3-(N-tetradecyl-N,N-dimethyl-ammonio)-propane-1-sulfonate (SB14) [1], available from Sigma as SB3-14.
References
- Zanna L and Haeuw JF. . pmid:16978932.
