SUME Buffer (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA) To make 100ml:
* 1.0ml 1M MOPS, pH6.8 stock solution * 1.0g SDS, or 10ml 10% SDS stock solution * 48.05g Urea * 2.0ml 0.5M EDTA stock solution
This is used for lysing yeast at pH6.8. For pH8 lyses, use the variation of SUME with Tris buffer known as SUTE.
from Yeast Protocols
- Zanna L and Haeuw JF. . pmid:16978932.