Drummond:Protocols: Difference between revisions
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*[[Drummond:DNA Prep|Yeast genomic DNA prep]] | *[[Drummond:DNA Prep|Yeast genomic DNA prep]] | ||
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*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | |||
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Revision as of 15:09, 12 March 2009
Local Protocols
Here are some protocols we use in the lab.
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- High-efficiency yeast transformation
- Yeast genomic DNA prep
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
