Drummond:Protocols: Difference between revisions
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*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | *[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | ||
*[[Drummond:Viability|Yeast OD and cell viability model]] | *[[Drummond:Viability|Yeast OD and cell viability model]] | ||
*[[Drummond:Pregrowth | *[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]] | ||
*[[Drummond:Transformation|High-efficiency yeast transformation]] | *[[Drummond:Transformation|High-efficiency yeast transformation]] | ||
*[[Drummond:DNA Prep|Yeast genomic DNA prep]] | *[[Drummond:DNA Prep|Yeast genomic DNA prep]] | ||
Revision as of 11:46, 1 May 2009
Local Protocols
Here are some protocols we use in the lab.
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- Standard conditions for flow cytometer growth rate assay
- High-efficiency yeast transformation
- Yeast genomic DNA prep
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
- Preparing anti-ubiquitin beads
- Periplasmic protein prep from E. coli
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
