Drummond:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
Dadrummond (talk | contribs) |
||
Line 4: | Line 4: | ||
Here are some protocols we use in the lab. | Here are some protocols we use in the lab. | ||
*[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]] | |||
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | *[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | ||
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | *[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] |
Revision as of 07:00, 15 July 2011
Local Protocols
Here are some protocols we use in the lab.
- Chloroform/methanol precipitation of proteins
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- Standard conditions for flow cytometer growth rate assay
- High-efficiency yeast transformation
- Yeast genomic DNA prep
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
- Preparing anti-ubiquitin beads
- Periplasmic protein prep from E. coli
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)