Drummond:Protocols

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(Local Protocols)
Current revision (10:47, 13 October 2012) (view source)
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==Local Protocols==
==Local Protocols==
Here are some protocols we use in the lab.
Here are some protocols we use in the lab.
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*[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]]
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*[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]]
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*[[Drummond:Lysis|Thoughts on lysis of yeast cells]]
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*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]]
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]]
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*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]]
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*[[Drummond:Fluorescence|Cell preparation and protocol to view RFP/TFP fluorescence]]
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*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]]
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*[[Drummond:Viability|Yeast OD and cell viability model]]
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*[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]]
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*[[Drummond:Transformation|High-efficiency yeast transformation]]
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*[[Drummond:DNA Prep|Yeast genomic DNA prep]] (consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead)
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*[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]]
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*[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]]
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*[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]]
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*[[Drummond:Periplasmic Prep|Periplasmic protein prep from <i>E. coli</i>]]
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==Remote Protocols==
==Remote Protocols==
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[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]
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*[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)]
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*[http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)]
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*[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)]
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==Data Analysis Protocols==
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*[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]]
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==Notes==
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*[[Drummond:PopGen|The relationship between the selection coefficient and relative growth rates: under construction]]
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*[[Drummond:Lysis|Thoughts on lysis of yeast cells]]
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Local Protocols

Here are some protocols we use in the lab.

Remote Protocols

Data Analysis Protocols

Notes

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