Drummond:Protocols
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Current revision (10:47, 13 October 2012) (view source) (rather rapid alternative) |
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==Local Protocols== | ==Local Protocols== | ||
Here are some protocols we use in the lab. | Here are some protocols we use in the lab. | ||
| - | *[[Drummond: | + | *[[Drummond:ChlorMethExtraction|Chloroform/methanol precipitation of proteins]] |
| - | + | ||
*[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | *[[Drummond:Ubiquitin_Western|Resolving and detecting ubiquitin]] | ||
*[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | *[[Drummond:Paromomycin|Using paromomycin to alter translation in yeast]] | ||
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*[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | *[[Drummond:Confocal|Using the Zeiss LSM 510 confocal microscope]] | ||
*[[Drummond:Viability|Yeast OD and cell viability model]] | *[[Drummond:Viability|Yeast OD and cell viability model]] | ||
| - | *[[ | + | *[[Drummond:Pregrowth| Standard conditions for flow cytometer growth rate assay]] |
| - | *[[ | + | *[[Drummond:Transformation|High-efficiency yeast transformation]] |
| + | *[[Drummond:DNA Prep|Yeast genomic DNA prep]] (consider the [http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html rather rapid yeast genomic DNA prep] instead) | ||
| + | *[[Drummond:Protein Isolation|Isolating total, soluble and insoluble protein fractions]] | ||
| + | *[[Drummond:Competent Cells|Making chemically competent <i>E. coli</i> cells]] | ||
| + | *[[Drummond:Anti-Ubiquitin Beads|Preparing anti-ubiquitin beads]] | ||
| + | *[[Drummond:Periplasmic Prep|Periplasmic protein prep from <i>E. coli</i>]] | ||
| + | <!-- *[[Drummond:Solubility|Extracting soluble and insoluble protein fractions]] --> | ||
==Remote Protocols== | ==Remote Protocols== | ||
| - | [http:// | + | *[http://depts.washington.edu/younglab/yeastprotocols(htm)/sporeenrichment.htm Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)] |
| - | [http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | + | *[http://labs.fhcrc.org/gottschling/Yeast%20Protocols/qgprep.html Rather rapid yeast genomic DNA prep (Gottschling Lab)] |
| + | *[http://genetics.mgh.harvard.edu/szostakweb/resources/Public%20Protocols/protein_page/index.html SDS-PAGE (Szostak Lab)] | ||
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| + | ==Data Analysis Protocols== | ||
| + | *[[Drummond:Akashi's Test|Akashi's test for translational accuracy selection]] | ||
| + | |||
| + | ==Notes== | ||
| + | *[[Drummond:PopGen|The relationship between the selection coefficient and relative growth rates: under construction]] | ||
| + | *[[Drummond:Lysis|Thoughts on lysis of yeast cells]] | ||
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</div> | </div> | ||
{{Drummond_Bottom}} | {{Drummond_Bottom}} | ||
Current revision
Local Protocols
Here are some protocols we use in the lab.
- Chloroform/methanol precipitation of proteins
- Resolving and detecting ubiquitin
- Using paromomycin to alter translation in yeast
- Cell preparation and protocol to view RFP/TFP fluorescence
- Using the Zeiss LSM 510 confocal microscope
- Yeast OD and cell viability model
- Standard conditions for flow cytometer growth rate assay
- High-efficiency yeast transformation
- Yeast genomic DNA prep (consider the rather rapid yeast genomic DNA prep instead)
- Isolating total, soluble and insoluble protein fractions
- Making chemically competent E. coli cells
- Preparing anti-ubiquitin beads
- Periplasmic protein prep from E. coli
Remote Protocols
- Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
- Rather rapid yeast genomic DNA prep (Gottschling Lab)
- SDS-PAGE (Szostak Lab)
